- Title
- Mass Spectrometry Reveals New Insights into the Production of Superoxide Anions and 4-Hydroxynonenal Adducted Proteins in Human Sperm
- Creator
- Netherton, Jacob Kendal; Hetherington, Louise; Ogle, Rachel Anne; Gavgani, Marum Mazloumi; Velkov, Tony; Villaverde, Ana Izabel Bilbin; Tanphaichitr, Nuch; Baker, Mark Andrew
- Relation
- Proteomics Vol. 20, Issue 2, no. 1900205
- Publisher Link
- http://dx.doi.org/10.1002/pmic.201900205
- Publisher
- Wiley-Blackwell
- Resource Type
- journal article
- Date
- 2020
- Description
- The free-radical theory of male infertility suggests that reactive oxygen species produced by the spermatozoa themselves are a leading cause of sperm dysfunction, including loss of sperm motility. However, the field is overshadowed on several fronts, primarily because: i) the probes used to measure reactive oxygen species (ROS) are imprecise; and ii) many reports suggesting that oxygen radicals are detrimental to sperm function add an exogenous source of ROS. Herein, a more reliable approach to measure superoxide anion production by human spermatozoa based on MS analysis is used. Furthermore, the formation of the lipid-peroxidation product 4-hydroxynonenal (4-HNE) during in vitro incubation using proteomics is also investigated. The data demonstrate that neither superoxide anion nor other free radicals that cause 4-HNE production are related to the loss of sperm motility during incubation. Interestingly, it appears that many of the 4-HNE adducted proteins, found within spermatozoa, originate from the prostate. A quantitative SWATH analysis demonstrate that these proteins transiently bind to sperm and are then shed during in vitro incubation. These proteomics-based findings propose a revised understanding of oxidative stress within the male reproductive tract.
- Subject
- 4-hydroxynonenal; proteomics; reactive oxygen species; spermatozoa; swath
- Identifier
- http://hdl.handle.net/1959.13/1442326
- Identifier
- uon:41655
- Identifier
- ISSN:1615-9853
- Language
- eng
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